Isolation of saturated alkylresorcinols from rye grains by countercurrent chromatography

Isolation of flavanol-anthocyanin adducts by countercurrent chromatography.

Pigments of the flavanol-anthocyanin (F-A+) type detected earlier in wine are synthesized using a protocol adapted from the synthesis of procyanidin dimers. The F-A+ adduct thus obtained is purified by countercurrent liquid-liquid partition, currently referred to as countercurrent chromatography (CCC). The solvent system consists of tert-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5,...

Preparative Isolation and Purification of Two Coumarins from Edgeworthia chrysantha Lindl by High Speed Countercurrent Chromatography

Two bioactive coumarins including 7-hydroxycoumarin (umbelliferone) and 7-hydroxyl-6-methoxy-3, 70-dicoumarylether (daphnoretin) were separated from the ethyl acetate extract of the stems of Edgeworthia chrysantha Lindl (E. papyrifera) by high speed countercurrent chromatography (HSCCC). The two-phase solvent system used was composed of n-hexane-ethyl acetate–methanol–water at an optimized volu...

Countercurrent Chromatography and High Speed Countercurrent Chromatography: Instrumentation

Engelsma M, KokWT and Smit HC (1990) Selective determination of trace levels of phenol in river water using electrochemical concentration modulation. Journal of Chromatography 506: 201}210. Fister JC, Jacobson C and RamseyM (1999) Ultra sensitive cross correlation electrophoresis on microdevices. Analytical Chemistry 71: 4460}4464. Kaljurand M and KuK llik E (1989) Computerized Multiple Input C...

Spiral countercurrent chromatography.

For many years, high-speed countercurrent chromatography conducted in open tubing coils has been widely used for the separation of natural and synthetic compounds. In this method, the retention of the stationary phase is solely provided by the Archimedean screw effect by rotating the coiled column in the centrifugal force field. However, the system fails to retain enough of the stationary phase...

Isolation of octopamine from annual rye grass.